In recent years, more and more attention has been given to the urgency for sustainable cities. population growth is causing cities to expand in a fast and uncontrolled way. we can observe that by the increasing number of traffic congestion in the big urban centres. this is proven to cause not only environmental problems, but also healthy issues in the population. private cars are still the most used transport mode worldwide, because, mainly in the emerging countries, there is no other competitive transport mode. even in developed countries, where public transport normally meets population’s necessity, there is the first and last mile problem, which does not always allow us to consider this system as a competitive alternative to the use of private vehicles. within this context, bicycle and public transport combination has been found as a competitive alternative to the use of private cars, since it facilitates the transport door-to-door. in this research, we conducted some analyses regarding the bicycle transit combination in the amsterdam region, focusing on how cyclists’ behaviour regarding their choice of how to access the public transport system by bicycle changed due to this big network change with the new metro line (the north-south line (nsl) which crosses amsterdam from south to north. from the analyses we could see an increase on the choice of accessing the metro, instead of the tram or bus. besides, as an indication of transportation mode substitutability, the higher the trip duration of the other pt modes (bus and tram), the higher the likelihood of a user to choose metro. it is also worth mentioning that a dummy variable for the time after the nsl line was in operation was estimated with a positive and significant effect, which ratifies that this new metro infrastructure increased the odds of a bicycle user to opt to ride her bike to a pt station and commute to a metro service.